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SUPV3L1-HA G207V
(Plasmid #232349)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232349 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTwist CMV
  • Total vector size (bp) 6569
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SUPV3L1
  • Species
    H. sapiens (human)
  • Mutation
    G207V
  • Entrez Gene
    SUPV3L1 (a.k.a. SUV3)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The G207V mutant is catalytically dead e.g. no helicase activity. See:
Minczuk M, et al. Nucleic Acids Res. 2002 Dec 1;30(23):5074-86. doi: https://doi.org/10.1093/nar/gkf647. PMID: 12466530; PMCID: PMC137961.
Szczesny RJ, et al. Nucleic Acids Res. 2010 Jan;38(1):279-98. doi: https://doi.org/10.1093/nar/gkp903. Epub 2009 Oct 28. PMID: 19864255; PMCID: PMC2800237.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SUPV3L1-HA G207V was a gift from Samantha Lewis (Addgene plasmid # 232349 ; http://n2t.net/addgene:232349 ; RRID:Addgene_232349)

  • For your References section:

    A spatial atlas of mitochondrial gene expression reveals dynamic translation hubs and remodeling in stress. Begeman A, Smolka JA, Shami A, Waingankar TP, Lewis SC. bioRxiv [Preprint]. 2024 Aug 17:2024.08.05.604215. doi: 10.1101/2024.08.05.604215. 10.1101/2024.08.05.604215 PubMed 39149346
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