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pTwist-CMV-HDGFL2_Cryptic_TwinstrepTEV_6xHis
(Plasmid #232344)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232344 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTwist CMV
  • Backbone manufacturer
    Twist Bioscience
  • Backbone size w/o insert (bp) 4197
  • Total vector size (bp) 6504
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HDGF Like 2
  • Alt name
    Hepatoma-Derived Growth Factor-Related Protein 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2307
  • Mutation
    TDP-43 regulated cryptic exon
  • Entrez Gene
    HDGFL2 (a.k.a. HDGF-2, HDGF2, HDGFRP2, HRP-2, HRP2)
  • Tags / Fusion Proteins
    • Twin-strep, TEV protease site (N terminal on insert)
    • GGGS linker, 6xHis (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTwist-CMV-HDGFL2_Cryptic_TwinstrepTEV_6xHis was a gift from Philip Wong (Addgene plasmid # 232344 ; http://n2t.net/addgene:232344 ; RRID:Addgene_232344)

  • For your References section:

    A fluid biomarker reveals loss of TDP-43 splicing repression in presymptomatic ALS-FTD. Irwin KE, Jasin P, Braunstein KE, Sinha IR, Garret MA, Bowden KD, Chang K, Troncoso JC, Moghekar A, Oh ES, Raitcheva D, Bartlett D, Miller T, Berry JD, Traynor BJ, Ling JP, Wong PC. Nat Med. 2024 Feb;30(2):382-393. doi: 10.1038/s41591-023-02788-5. Epub 2024 Jan 26. 10.1038/s41591-023-02788-5 PubMed 38278991
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