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Addgene

pLenti-mEtfdh-AAA4
(Plasmid #232316)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232316 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUltra
  • Vector type
    Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Etfdh
  • Alt name
    Etfqo
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1863
  • Mutation
    PAM Sequence Mutations: (G36C; T39C, C42T; C72T, T78A, G81A, T84C, T87G); Insertion: 4 (AAA) tracks (115 to 126)
  • Entrez Gene
    Etfdh (a.k.a. 0610010I20Rik, AV001013)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see Addgene plasmid #24129 for pUltra cloning details.

Please visit https://doi.org/10.1101/2024.10.25.620155 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-mEtfdh-AAA4 was a gift from Ivan Topisirovic (Addgene plasmid # 232316 ; http://n2t.net/addgene:232316 ; RRID:Addgene_232316)

  • For your References section:

    Mitochondrial ETF insufficiency drives neoplastic growth by selectively optimizing cancer bioenergetics. Papadopoli D, Palia R, Jovanovic P, Tabariès S, Ciccolini E, Sabourin V, Igelmann S, McLaughlan S, Zhan L, Kim H, Chekkal N, Szkop KJ, Bertomeu T, Zeng J, Vassalakis J, Afzali F, Mzoughi S, Guccione E, Tyers M, Avizonis D, Larsson O, Postovit LM, Djuranovic S, Ursini-Siegel J, Siegel PM, Pollak M, Topisirovic I. bioRxiv 2024.10.25.620155 10.1101/2024.10.25.620155
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