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pET-Octo_VirD2_NLS-Cas9-6xHis
(Plasmid #232296)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232296 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-NLS-Cas9-6xHis (Addgene Plasmid #62934)
  • Backbone manufacturer
    David Liu
  • Backbone size w/o insert (bp) 5140
  • Total vector size (bp) 9286
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For protein expression, transform into BL21(DE3) cells. Induce at 0.6 OD600 with 0.5 mM IPTG (working concentration). Grow overnight (16 h) at 20–25 °C.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Octo_VirD2_NLS-Cas9-6xHis
  • Alt name
    DN Cas9
  • Species
    Synthetic
  • Insert Size (bp)
    4185
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis (C terminal on insert)
    • Octopine-type VirD2-derived NLS (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-Octo_VirD2_NLS-Cas9-6xHis was a gift from Hee-Sik Kim (Addgene plasmid # 232296 ; http://n2t.net/addgene:232296 ; RRID:Addgene_232296)

  • For your References section:

    Cas9-mediated gene-editing frequency in microalgae is doubled by harnessing the interaction between importin alpha and phytopathogenic NLSs. Le TT, Choi HI, Kim JW, Yun JH, Lee YH, Jeon EJ, Kwon KK, Cho DH, Choi DY, Park SB, Yoon HR, Lee J, Sim EJ, Lee YJ, Kim HS. Proc Natl Acad Sci U S A. 2025 Mar 11;122(10):e2415072122. doi: 10.1073/pnas.2415072122. Epub 2025 Mar 3. 10.1073/pnas.2415072122 PubMed 40030016
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