pPMS-2284
(Plasmid
#232293)
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PurposeProtein Expression
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Depositing Lab
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Publication
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232293 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRK5
- Backbone size w/o insert (bp) 4800
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHS69
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Alt nameAnti-Homer Nanobody
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SpeciesL. glama (llama) and Guinea Pig
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Insert Size (bp)600
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site AscI (unknown if destroyed)
Resource Information
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A portion of this plasmid was derived from a plasmid made byJames Trimmer, Addgene plasmid #134716
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPMS-2284 was a gift from Jeremy Nathans (Addgene plasmid # 232293 ; http://n2t.net/addgene:232293 ; RRID:Addgene_232293)