Skip to main content
Addgene

pPMS-1852
(Plasmid #232287)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232287 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pADL-10b
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Phage Display Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fn3 domain #10
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    300
  • Mutation
    D7K
  • Tag / Fusion Protein
    • PreScission Cleavage, HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPMS-1852 was a gift from Jeremy Nathans (Addgene plasmid # 232287 ; http://n2t.net/addgene:232287 ; RRID:Addgene_232287)
Related items: