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pcDNA3.1 FFSS-BACH1 C122E
(Plasmid #232274)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232274 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 8500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BACH1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2600
  • Mutation
    changed cysteine 122 to glutamic acid
  • Entrez Gene
    BACH1 (a.k.a. BACH-1, BTBD24)
  • Promoter CMV
  • Tag / Fusion Protein
    • 2xFLAG-2xSTREP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 FFSS-BACH1 C122E was a gift from Michele Pagano (Addgene plasmid # 232274 ; http://n2t.net/addgene:232274 ; RRID:Addgene_232274)

  • For your References section:

    Recognition of BACH1 quaternary structure degrons by two F-box proteins under oxidative stress. Cao S, Garcia SF, Shi H, James EI, Kito Y, Shi H, Mao H, Kaisari S, Rona G, Deng S, Goldberg HV, Ponce J, Ueberheide B, Lignitto L, Guttman M, Pagano M, Zheng N. Cell. 2024 Oct 30:S0092-8674(24)01188-7. doi: 10.1016/j.cell.2024.10.012. 10.1016/j.cell.2024.10.012 PubMed 39504958
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