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Addgene

pGEX6P1 human RLIM N581K
(Plasmid #232241)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232241 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX6P1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositing lab recommends Stellar competent cells for propagation.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GST-human RLIM N581K
  • Alt name
    RNF12
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1872
  • Mutation
    N581K
  • Entrez Gene
    RLIM (a.k.a. MRX61, NY-REN-43, RNF12, TOKAS)
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX6P1 human RLIM N581K was a gift from Francisco Bustos (Addgene plasmid # 232241 ; http://n2t.net/addgene:232241 ; RRID:Addgene_232241)

  • For your References section:

    RLIM-specific activity reporters define variant pathogenicity in Tonne-Kalscheuer syndrome. Bandi V, Rennie M, Koch I, Gill P, Pacheco OD, Berg AD, Cui H, Ward DI, Bustos F. HGG Adv. 2024 Oct 31;6(1):100378. doi: 10.1016/j.xhgg.2024.100378. 10.1016/j.xhgg.2024.100378 PubMed 39482882
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