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Addgene

pCAGGS puro GFP-REX1-IRES-mCherry
(Plasmid #232239)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232239 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS puro
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositing lab recommends Stellar competent cells for propagation.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    REX1-GFP-IRES-mCherry
  • Alt name
    ZFP42
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    864
  • Entrez Gene
    Zfp42 (a.k.a. Rex, Rex-, Rex-1, Rex1, Zfp-4, Zfp-42)
  • Tag / Fusion Protein
    • AcGFP1 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS puro GFP-REX1-IRES-mCherry was a gift from Francisco Bustos (Addgene plasmid # 232239 ; http://n2t.net/addgene:232239 ; RRID:Addgene_232239)

  • For your References section:

    RLIM-specific activity reporters define variant pathogenicity in Tonne-Kalscheuer syndrome. Bandi V, Rennie M, Koch I, Gill P, Pacheco OD, Berg AD, Cui H, Ward DI, Bustos F. HGG Adv. 2024 Oct 31;6(1):100378. doi: 10.1016/j.xhgg.2024.100378. 10.1016/j.xhgg.2024.100378 PubMed 39482882
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