Skip to main content
Addgene

pRExT2A-CT-BmSc
(Plasmid #232208)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232208 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUC57 simple
  • Vector type
    Bacterial Expression
  • Selectable markers
    Blasticidin ; in Trypanosoma brucei

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mScarlet
  • Species
    Synthetic

Cloning Information

  • Cloning method Gene Synthesis

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid template contains no kinetoplastid-specific sequences, so it is fully compatible with mammalian and other eukaryotic systems.

Please visit https://doi.org/10.1101/2024.11.02.621556 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRExT2A-CT-BmSc was a gift from Calvin Tiengwe (Addgene plasmid # 232208 ; http://n2t.net/addgene:232208 ; RRID:Addgene_232208)
  • For your References section:

    A high-throughput protein tagging toolkit that retains endogenous UTRs for studying gene regulation in Kinetoplastids. Carbajo CG, Han X, Savur B, Upadhyaya A, Taha F, Tinti M, Wheeler RJ, Yates PA, Tiengwe C. bioRxiv [Preprint]. 2024 Nov 3:2024.11.02.621556. doi: 10.1101/2024.11.02.621556. 10.1101/2024.11.02.621556 PubMed 39554005