pRExT2A-CT-BmSc
(Plasmid
#232208)
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PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-BSD-3xTy
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232208 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57 simple
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Vector typeBacterial Expression
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Selectable markersBlasticidin ; in Trypanosoma brucei
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemScarlet
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SpeciesSynthetic
Cloning Information
- Cloning method Gene Synthesis
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid template contains no kinetoplastid-specific sequences, so it is fully compatible with mammalian and other eukaryotic systems.
Please visit https://doi.org/10.1101/2024.11.02.621556 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRExT2A-CT-BmSc was a gift from Calvin Tiengwe (Addgene plasmid # 232208 ; http://n2t.net/addgene:232208 ; RRID:Addgene_232208) -
For your References section:
A high-throughput protein tagging toolkit that retains endogenous UTRs for studying gene regulation in Kinetoplastids. Carbajo CG, Han X, Savur B, Upadhyaya A, Taha F, Tinti M, Wheeler RJ, Yates PA, Tiengwe C. bioRxiv [Preprint]. 2024 Nov 3:2024.11.02.621556. doi: 10.1101/2024.11.02.621556. 10.1101/2024.11.02.621556 PubMed 39554005