mP_ILB_Vector 1
(Plasmid
#232193)
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Purpose(Empty Backbone) The plasmid vector designed for the expression of four genes in oleaginous yeasts, including Yarrowia lipolytica and Rhodotorula toruloides.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 232193 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $400 * | |
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Backbone
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Vector backboneDesigned and synthesized
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Modifications to backboneThe backbone of vector contains: Ori, AmpR, 26s rDNA UP-Arm, 26s rDNA Down-Arm, four TEF2 promoter with intron, URA3 franked with loxP, four Xpr2 terminator.
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Vector typeYeast Expression, Synthetic Biology
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mP_ILB_Vector 1 was a gift from Matrika Bhattarai (Addgene plasmid # 232193 ; http://n2t.net/addgene:232193 ; RRID:Addgene_232193)
Map uploaded by the depositor.
