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Addgene

Tol2-mfap4-BFP
(Plasmid #232188)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232188 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Tol2
  • Backbone size w/o insert (bp) 3100
  • Vector type
    Zebrafish Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BFP
  • Insert Size (bp)
    696
  • Promoter mfap4

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer cgccattttgtctcgtattg
  • 3′ sequencing primer GTGGTTTGTCCAAACTCATCA
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    mfap4 promoter amplified from p5E-mfap4 (Addgene catalog no. 70052, a gift from David Tobin) BFP promoter amplified from Tol2-lyz-BFP (PMID 30071103)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tol2-mfap4-BFP was a gift from Emily Rosowski (Addgene plasmid # 232188 ; http://n2t.net/addgene:232188 ; RRID:Addgene_232188)

  • For your References section:

    Glucocorticoids Suppress NF-kappaB-Mediated Neutrophil Control of Aspergillus fumigatus Hyphal Growth. Thrikawala SU, Anderson MH, Rosowski EE. J Immunol. 2024 Oct 1;213(7):971-987. doi: 10.4049/jimmunol.2400021. 10.4049/jimmunol.2400021 PubMed 39178124
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