pLV-U6-gRNA scaffold-UbC-mCherry-PuroR
(Plasmid
#232183)
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Purpose(Empty Backbone) Lentiviral plasmid for cloning an spCas9 gRNA with puromycin resistance and mCherry
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232183 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFUGW
- Backbone size (bp) 12986
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Vector typeMammalian Expression, Lentiviral
- Promoter U6, hUbC
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Selectable markersPuromycin ; mCherry
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.03.03.583177 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-U6-gRNA scaffold-UbC-mCherry-PuroR was a gift from Charles Gersbach (Addgene plasmid # 232183 ; http://n2t.net/addgene:232183 ; RRID:Addgene_232183) -
For your References section:
Activation of the imprinted Prader-Willi syndrome locus by CRISPR-based epigenome editing. Rohm D, Black JB, McCutcheon SR, Barrera A, Berry SS, Morone DJ, Nuttle X, de Esch CE, Tai DJC, Talkowski ME, Iglesias N, Gersbach CA. Cell Genom. 2025 Feb 12;5(2):100770. doi: 10.1016/j.xgen.2025.100770. 10.1016/j.xgen.2025.100770 PubMed 39947136