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pLV-U6-gRNA scaffold-UbC-mCherry-PuroR
(Plasmid #232183)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232183 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUGW
  • Backbone size (bp) 12986
  • Vector type
    Mammalian Expression, Lentiviral
  • Promoter U6, hUbC
  • Selectable markers
    Puromycin ; mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.03.03.583177 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-U6-gRNA scaffold-UbC-mCherry-PuroR was a gift from Charles Gersbach (Addgene plasmid # 232183 ; http://n2t.net/addgene:232183 ; RRID:Addgene_232183)

  • For your References section:

    Activation of the imprinted Prader-Willi syndrome locus by CRISPR-based epigenome editing. Rohm D, Black JB, McCutcheon SR, Barrera A, Berry SS, Morone DJ, Nuttle X, de Esch CE, Tai DJC, Talkowski ME, Iglesias N, Gersbach CA. Cell Genom. 2025 Feb 12;5(2):100770. doi: 10.1016/j.xgen.2025.100770. 10.1016/j.xgen.2025.100770 PubMed 39947136
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