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Addgene

pLV-UbC-VP64-dCas9-VP64
(Plasmid #232179)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232179 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUGW
  • Backbone size w/o insert (bp) 9600
  • Total vector size (bp) 14289
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    VP64-dCas9-VP64
  • Species
    H. sapiens (human); S. pyogenes
  • Insert Size (bp)
    4400
  • Mutation
    D10A, H840A
  • Promoter hUbC

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Addgene plasmid #59791 modified to replace GFP with BSD

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.03.03.583177 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-UbC-VP64-dCas9-VP64 was a gift from Charles Gersbach (Addgene plasmid # 232179 ; http://n2t.net/addgene:232179 ; RRID:Addgene_232179)

  • For your References section:

    Activation of the imprinted Prader-Willi syndrome locus by CRISPR-based epigenome editing. Rohm D, Black JB, McCutcheon SR, Barrera A, Berry SS, Morone DJ, Nuttle X, de Esch CE, Tai DJC, Talkowski ME, Iglesias N, Gersbach CA. Cell Genom. 2025 Feb 12;5(2):100770. doi: 10.1016/j.xgen.2025.100770. 10.1016/j.xgen.2025.100770 PubMed 39947136
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