p5954 pcDNA-Xpress-His-USP15 C269A
(Plasmid #23217)

• Depositing Lab: Peter Howley
• Publication: Vos et al J Virol. 2009 Sep . 83(17):8885-92.

Backbone

• Vector backbone: pcDNA-4C
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5300
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: USP15
• Alt name: ubiquitin carboxyl-terminal hydrolase 15 isoform 2
• Species: H. sapiens (human)
• Insert Size (bp): 702
• Mutation: C269A
• GenBank ID: NP_006304.1
• Entrez Gene: USP15 (a.k.a. UNPH-2, UNPH4)
• Tags / Fusion Proteins:
  • His (N terminal on backbone)
  • Xpress (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry