Cox4-DsRed retrovirus
(Plasmid #23215)

• Depositing Lab: David Chan
• Publication: Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200.

Backbone

• Vector backbone: Moloney retrovirus expression plasmid
• Backbone size w/o insert (bp): 5000
• Vector type: Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cox4
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 700
• Mutation: Cox4 mitochondrial targeting sequence fused to DsRed variant
• Entrez Gene: COX4 (a.k.a. YGL187C)
• Tag / Fusion Protein:
  • DsRed (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: DsRed1-N

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry