MPS1 Human
(Plasmid
#232149)
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PurposeMPS1 from Human codon optimized for S. cerevisiae in pUCIDT
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232149 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUCIDT(AMP)
- Total vector size (bp) 5338
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMPS1
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SpeciesH. sapiens (human)
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Insert Size (bp)2586
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Entrez GeneTTK (a.k.a. CT96, ESK, MPH1, MPS1, MPS1L1, PYT)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer M13F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MPS1 Human was a gift from Mark Winey (Addgene plasmid # 232149 ; http://n2t.net/addgene:232149 ; RRID:Addgene_232149) -
For your References section:
Spindle checkpoint activation by fungal orthologs of the S. cerevisiae Mps1 kinase. Fabritius A, Alonso A, Wood A, Sulthana S, Winey M. PLoS One. 2024 Mar 26;19(3):e0301084. doi: 10.1371/journal.pone.0301084. eCollection 2024. 10.1371/journal.pone.0301084 PubMed 38530809