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Addgene

pME-zdgat2-CDS-FLAG
(Plasmid #232142)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232142 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR-221
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4672
  • Vector type
    Gateway cloning middle entry

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dgat2
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1106
  • Entrez Gene
    dgat2 (a.k.a. MGC113394, zgc:113394)
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME-zdgat2-CDS-FLAG was a gift from Steven Farber (Addgene plasmid # 232142 ; http://n2t.net/addgene:232142 ; RRID:Addgene_232142)

  • For your References section:

    Zebrafish are resilient to the loss of major diacylglycerol acyltransferase enzymes. Wilson MH, Hensley MR, Shen MC, Lu HY, Quinlivan VH, Busch-Nentwich EM, Rawls JF, Farber SA. J Biol Chem. 2024 Nov 6;300(12):107973. doi: 10.1016/j.jbc.2024.107973. 10.1016/j.jbc.2024.107973 PubMed 39510175
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