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pDEST-CMV-zdgat2FLAG-SV40
(Plasmid #232141)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232141 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    394 pDEST Tol2pA2
  • Backbone manufacturer
    Invitrogen via Chien Lab
  • Backbone size w/o insert (bp) 5883
  • Vector type
    zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dgat2
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    2592
  • Entrez Gene
    dgat2 (a.k.a. MGC113394, zgc:113394)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Some components were from the Chien Lab Tol2 kit

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST-CMV-zdgat2FLAG-SV40 was a gift from Steven Farber (Addgene plasmid # 232141 ; http://n2t.net/addgene:232141 ; RRID:Addgene_232141)

  • For your References section:

    Zebrafish are resilient to the loss of major diacylglycerol acyltransferase enzymes. Wilson MH, Hensley MR, Shen MC, Lu HY, Quinlivan VH, Busch-Nentwich EM, Rawls JF, Farber SA. J Biol Chem. 2024 Nov 6;300(12):107973. doi: 10.1016/j.jbc.2024.107973. 10.1016/j.jbc.2024.107973 PubMed 39510175
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