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Addgene

pCRII-dgat2 in situ probe
(Plasmid #232134)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232134 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCRII-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3900
  • Vector type
    in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dgat2
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    786
  • Entrez Gene
    dgat2 (a.k.a. MGC113394, zgc:113394)
  • Promoter SP6/T7

Cloning Information

  • Cloning method TOPO Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRII-dgat2 in situ probe was a gift from Steven Farber (Addgene plasmid # 232134 ; http://n2t.net/addgene:232134 ; RRID:Addgene_232134)

  • For your References section:

    Zebrafish are resilient to the loss of major diacylglycerol acyltransferase enzymes. Wilson MH, Hensley MR, Shen MC, Lu HY, Quinlivan VH, Busch-Nentwich EM, Rawls JF, Farber SA. J Biol Chem. 2024 Nov 6;300(12):107973. doi: 10.1016/j.jbc.2024.107973. 10.1016/j.jbc.2024.107973 PubMed 39510175
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