JDW 1035 (pME_V5_TCF7L1_P2A_H2A_mCherry)
(Plasmid
#232125)
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PurposeA middle entry gateway clone containing V5 tagged human TCFL7 / TCF3 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232125 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR 221
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Vector typeGateway cloning
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTCF7L1 / TCF3
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SpeciesH. sapiens (human)
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Entrez GeneTCF7L1 (a.k.a. TCF-3, TCF3)
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Tag
/ Fusion Protein
- V5 (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
JDW 1035 (pME_V5_TCF7L1_P2A_H2A_mCherry) was a gift from Joshua Wythe (Addgene plasmid # 232125 ; http://n2t.net/addgene:232125 ; RRID:Addgene_232125) -
For your References section:
Defining the Transcriptional and Epigenetic Basis of Organotypic Endothelial Diversity in the Developing and Adult Mouse. Cantu Gutierrez ME, Hill MC, Largoza G, Martin JF, Wythe JD. bioRxiv 2021.11.15.468651 10.1101/2021.11.15.468651