Mfn1-Myc
(Plasmid #23212)

• Depositing Lab: David Chan
• Publication: Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200.

Backbone

• Vector backbone: pcDNA3.1(-)/Myc-His
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Mfn1
• Species: M. musculus (mouse)
• Entrez Gene: Mfn1 (a.k.a. 2310002F04Rik, 6330416C07Rik, D3Ertd265e, HR2, mKIAA4032)
• Promoter: CMV
• Tags / Fusion Proteins:
  • 9xMyc (C terminal on insert)
  • Myc (C terminal on backbone)
  • 6xHis (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (destroyed during cloning)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 19 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry