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pET28 MBP-TEV-Rat BCKDK-His
(Plasmid #232119)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232119 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pet28
  • Backbone size w/o insert (bp) 5239
  • Total vector size (bp) 7600
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    BCKDK
  • Alt name
    Branched-chain alpha-ketoacid dehydrogenase kinase
  • Alt name
    NP_062117.2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    2349
  • Entrez Gene
    Bckdk (a.k.a. BDK)
  • Promoter T7 Promoter
  • Tags / Fusion Proteins
    • Maltose-Binding Protein (MBP) (N terminal on insert)
    • Tev Site (N terminal on insert)
    • 6 x His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer T7 Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28 MBP-TEV-Rat BCKDK-His was a gift from Ruth Sommese (Addgene plasmid # 232119 ; http://n2t.net/addgene:232119 ; RRID:Addgene_232119)

  • For your References section:

    Structural studies identify angiotensin II receptor blocker-like compounds as branched-chain ketoacid dehydrogenase kinase inhibitors. Liu S, Kormos BL, Knafels JD, Sahasrabudhe PV, Rosado A, Sommese RF, Reyes AR, Ward J, Roth Flach RJ, Wang X, Buzon LM, Reese MR, Bhattacharya SK, Omoto K, Filipski KJ. J Biol Chem. 2023 Mar;299(3):102959. doi: 10.1016/j.jbc.2023.102959. Epub 2023 Jan 28. 10.1016/j.jbc.2023.102959 PubMed 36717078
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