Addgene: pETDuet-1 Flag-LplA Skip to main content
Addgene

pETDuet-1 Flag-LplA
(Plasmid #232118)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232118 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    petDuet-1
  • Backbone size w/o insert (bp) 5360
  • Total vector size (bp) 6416
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lplA
  • Alt name
    Lipoate-protein ligase A
  • Species
    Escherichia coli (strain K12)
  • Insert Size (bp)
    1041
  • Entrez Gene
    lplA (a.k.a. b4386, ECK4378, slr, yjjF)
  • Promoter T7 Promoter
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer T7 Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETDuet-1 Flag-LplA was a gift from Ruth Sommese (Addgene plasmid # 232118 ; http://n2t.net/addgene:232118 ; RRID:Addgene_232118)

  • For your References section:

    Structural studies identify angiotensin II receptor blocker-like compounds as branched-chain ketoacid dehydrogenase kinase inhibitors. Liu S, Kormos BL, Knafels JD, Sahasrabudhe PV, Rosado A, Sommese RF, Reyes AR, Ward J, Roth Flach RJ, Wang X, Buzon LM, Reese MR, Bhattacharya SK, Omoto K, Filipski KJ. J Biol Chem. 2023 Mar;299(3):102959. doi: 10.1016/j.jbc.2023.102959. Epub 2023 Jan 28. 10.1016/j.jbc.2023.102959 PubMed 36717078
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