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Addgene

pCas9-EcRT-Z3-Kan
(Plasmid #232096)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232096 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCas9-EcRT-GAL-Kan
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    Kanamycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Z3 promoter and Z3EV transcription factor
  • Species
    Synthetic
  • Promoter Z3

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    David Botstein, pRS416-yZ3EV-Z3pr (Addgene Plasmid #69099)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The GAL1-GAL10 promoter driving expression of SpCas9 and Ec86 RT in pCas9-EcRT-GAL-Kan was swapped with an estradiol-inducible Z3 promoter and the Z3EV transcription factor was inserterted into the Mph1103I site. Please visit https://doi.org/10.1101/2024.08.06.606807 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCas9-EcRT-Z3-Kan was a gift from Jeffrey Lewis (Addgene plasmid # 232096 ; http://n2t.net/addgene:232096 ; RRID:Addgene_232096)

  • For your References section:

    Improved vectors for retron-mediated CRISPR-Cas9 genome editing in Saccharomyces cerevisiae. Stuecker TN, Hood SE, Pineda JM, Lenaduwe S, Winter J, Sadhu MJ, Lewis JA. bioRxiv [Preprint]. 2024 Aug 7:2024.08.06.606807. doi: 10.1101/2024.08.06.606807. 10.1101/2024.08.06.606807 PubMed 39149293
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