Skip to main content
Addgene

pCas9-EcRT-GAL-Kan
(Plasmid #232092)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232092 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pAG26 (Addgene Plasmid # 35127)
  • Backbone manufacturer
    John McCusker
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    Kanamycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9 and Ec86 reverse transcriptase
  • Species
    S. pyogenes (Cas9) and E. coli (Ec86 RT)
  • Mutation
    Ec86 reverse transcriptase is codon optimized for yeast
  • Promoter GAL1-GAL10

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Hunter Fraser, pZS157 (Addgene Plasmid #114454)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SpCas9 and Ec86 RT were cloned into pAG26 using PfoI (5') and Mph1103I (3') sites (not destroyed during cloning), and HphMX marker was swapped for KanMX . Please visit https://doi.org/10.1101/2024.08.06.606807 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCas9-EcRT-GAL-Kan was a gift from Jeffrey Lewis (Addgene plasmid # 232092 ; http://n2t.net/addgene:232092 ; RRID:Addgene_232092)

  • For your References section:

    Improved vectors for retron-mediated CRISPR-Cas9 genome editing in Saccharomyces cerevisiae. Stuecker TN, Hood SE, Pineda JM, Lenaduwe S, Winter J, Sadhu MJ, Lewis JA. bioRxiv [Preprint]. 2024 Aug 7:2024.08.06.606807. doi: 10.1101/2024.08.06.606807. 10.1101/2024.08.06.606807 PubMed 39149293
Related items: