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Addgene

pMO819
(Plasmid #232072)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232072 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    3rd generation lentiviral backbone
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Depositors also recommend growing this plasmid in Stellar competent cells.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cytosine Base Editor
  • Alt name
    3xFLAG_NLS_APOBEC-1_Cas9(D10A)_UGI_NLS
  • Promoter EF-1-alpha core promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer tgatgtcgtgtactggctc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note Addgene NGS identified a single mutation in BleoR- E96K. Depositor does not use this selection marker. Unsure if this mutation will affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMO819 was a gift from Franz Meitinger (Addgene plasmid # 232072 ; http://n2t.net/addgene:232072 ; RRID:Addgene_232072)

  • For your References section:

    Genetic Engineering and Screening Using Base Editing and Inducible Gene Knockout. Ng EFY, Meitinger F. Methods Mol Biol. 2025;2872:167-187. doi: 10.1007/978-1-0716-4224-5_12. 10.1007/978-1-0716-4224-5_12 PubMed 39616576
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