pCDNA5 FRT TO FLAG LRRK1
(Plasmid
#232062)
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PurposeExpresses FLAG-LRRK1 in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232062 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDNA5
- Backbone size w/o insert (bp) 5118
- Total vector size (bp) 1163
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLRRK1
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SpeciesH. sapiens (human)
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Insert Size (bp)6045
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Entrez GeneLRRK1 (a.k.a. OSMD, RIPK6, Roco1)
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byMedical Research Council–Protein Phosphorylation and Ubiquitylation Unit (https://www.ppu.mrc.ac.uk/): pcDNA5-FRT/TO-FLAG LRRK1 wt
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid derived from pcDNA5-FRT/TO-FLAG LRRK1 wt from Medical Research Council–Protein Phosphorylation and Ubiquitylation Unit (https://www.ppu.mrc.ac.uk/)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA5 FRT TO FLAG LRRK1 was a gift from Samara Reck-Peterson (Addgene plasmid # 232062 ; http://n2t.net/addgene:232062 ; RRID:Addgene_232062)