pBFC0984
(Plasmid
#231992)
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PurposeCRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 231992 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep15A
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Vector typeBacterial Expression, CRISPR, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namedRfxCas13d
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Alt namedCas13d
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SpeciesRuminococcus flavefaciens
- Promoter pTet
Cloning Information for Gene/Insert 1
- Cloning method Golden Gate
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namecrRNA with 2xBsaI spacer
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SpeciesRuminococcus flavefaciens
- Promoter J23119
Cloning Information for Gene/Insert 2
- Cloning method Golden Gate
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.09.18.558157 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBFC0984 was a gift from Brady Cress (Addgene plasmid # 231992 ; http://n2t.net/addgene:231992 ; RRID:Addgene_231992) -
For your References section:
CRISPRi-ART enables functional genomics of diverse bacteriophages using RNA-binding dCas13d. Adler BA, Al-Shimary MJ, Patel JR, Armbruster EG, Colognori D, Charles EJ, Miller KV, Lahiri A, Cui ML, Oromi-Bosch A, Voelker A, Trinidad M, Lee J, Beurnier S, Boger R, Nomburg J, Barrangou R, Mutalik VK, Schoeniger JS, Pogliano JA, Savage DF, Doudna JA, Cress BF. Nat Microbiol. 2025 Mar;10(3):694-709. doi: 10.1038/s41564-025-01935-7. Epub 2025 Feb 26. 10.1038/s41564-025-01935-7 PubMed 40011704
Map uploaded by the depositor.
