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Addgene

TRE-pfv-Sapphire
(Plasmid #231957)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231957 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLIX403
  • Vector type
    Mammalian Expression, Lentiviral ; Doxocycline inducible
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    genetically encoded multimeric nanoparticles
  • Alt name
    GEM
  • Species
    Pyrococcus furiosus
  • Insert Size (bp)
    1816
  • GenBank ID
    AB214633.1
  • Promoter tight TRE promoter

Cloning Information

  • Cloning method Other
  • 5′ sequencing primer gatcgcctggagaattggctagcatgctctcaataaatccaaccc
  • 3′ sequencing primer gtggtggtggaccggttcatttgtacaattcatcaataccatg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.11.17.623896 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TRE-pfv-Sapphire was a gift from Michael Shtutman (Addgene plasmid # 231957 ; http://n2t.net/addgene:231957 ; RRID:Addgene_231957)

  • For your References section:

    Single Particle Tracking of Genetically Encoded Nanoparticles: Optimizing Expression for Cytoplasmic Diffusion Studies. Korunova E, Sikirzhystki V, Twiss JL, Vasquez P, Shtutman M. bioRxiv [Preprint]. 2024 Nov 18:2024.11.17.623896. doi: 10.1101/2024.11.17.623896. 10.1101/2024.11.17.623896 PubMed 39605363
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