pHR_TRE3G_mCherry_WPRE_PGK_tagBFP
(Plasmid
#231952)
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PurposeLentiviral vector for inducible expression of the red fluorescent protein mCherry (TRE3G promoter) and constitutive expression of the blue fluorescent protein tagBFP (PGK promoter)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 231952 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHR
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry
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SpeciesSynthetic
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer GCAGACTTTACTCCCTATC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHR_TRE3G_mCherry_WPRE_PGK_tagBFP was a gift from Leonardo Morsut (Addgene plasmid # 231952 ; http://n2t.net/addgene:231952 ; RRID:Addgene_231952)