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Addgene

pLKO.1 EF1a-EGFP
(Plasmid #231925)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231925 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO.1
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Species
    Aequorea victoria
  • Promoter EF-1a

Cloning Information

  • Cloning method Other

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    David Root, Plasmid pLKO.1 - TRC control (Addgene # 10879); Jennifer Doudna, Plasmid GFP-PS9 (Addgene # 177944)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO.1 EF1a-EGFP was a gift from Sriram Neelamegham (Addgene plasmid # 231925 ; http://n2t.net/addgene:231925 ; RRID:Addgene_231925)

  • For your References section:

    Tuning the tropism and infectivity of SARS-CoV-2 virus-like particles (VLPs) for mRNA delivery. Qi Y, Davidson B, Pajic P, Chen X, Omer G, Min G, Neelamegham S. Nucleic Acids Research, Volume 53, Issue 5, 24 March 2025, gkaf133 10.1093/nar/gkaf133
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