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Addgene

SARS-CoV spike all5KO mutant
(Plasmid #231921)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 231921 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    spike all5KO
  • Species
    SARS-CoV
  • Mutation
    N1080Q, N1116Q, N1140Q, N1155Q, N1176Q
  • Entrez Gene
    S (a.k.a. sars2, E2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • CD5 signal peptide (N terminal on insert)
    • C9 (C terminal on insert)

Cloning Information

  • Cloning method Other

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Fang Li, Plasmid pcDNA3.1-SARS-Spike (Addgene # 145031)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SARS-CoV spike all5KO mutant was a gift from Sriram Neelamegham (Addgene plasmid # 231921 ; http://n2t.net/addgene:231921 ; RRID:Addgene_231921)

  • For your References section:

    Conserved role of spike S2 domain N-glycosylation across betacoronaviruses. Yang Q, Kelkar A, Manicassamy B, Neelamegham S. npj Viruses. 2025 Jan; 3(4). doi: 10.1038/s44298-024-00085-7. 10.1038/s44298-024-00085-7
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