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Addgene

SARS-CoV-2 spike G145 mutant
(Plasmid #231918)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231918 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    spike G145
  • Species
    SARS-CoV-2
  • Mutation
    N1098Q, N1173Q, N1194Q
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Hyeryun Choe and Michael Farzan, Plasmid pCAGGS-SARS2-S-D614 (C-flag) (Addgene #156420)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SARS-CoV-2 spike G145 mutant was a gift from Sriram Neelamegham (Addgene plasmid # 231918 ; http://n2t.net/addgene:231918 ; RRID:Addgene_231918)

  • For your References section:

    Conserved role of spike S2 domain N-glycosylation across betacoronaviruses. Yang Q, Kelkar A, Manicassamy B, Neelamegham S. npj Viruses. 2025 Jan; 3(4). doi: 10.1038/s44298-024-00085-7. 10.1038/s44298-024-00085-7
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