Const.6 EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc-PS9
(Plasmid
#231910)
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PurposeFor packaging EGFP, dTomato, TagBFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 231910 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc
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SpeciesSynthetic
- Promoter CMV
Cloning Information
- Cloning method Other
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byKosuke Yusa, pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W (Addgene # 67974)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Const.6 EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc-PS9 was a gift from Sriram Neelamegham (Addgene plasmid # 231910 ; http://n2t.net/addgene:231910 ; RRID:Addgene_231910) -
For your References section:
Tuning the tropism and infectivity of SARS-CoV-2 virus-like particles (VLPs) for mRNA delivery. Qi Y, Davidson B, Pajic P, Chen X, Omer G, Min G, Neelamegham S. Nucleic Acids Research, Volume 53, Issue 5, 24 March 2025, gkaf133 10.1093/nar/gkaf133