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Addgene

LVDP 2P.2
(Plasmid #231904)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231904 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCSC-G
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nucleocapsid protein-T2A-membrane protein-IRES-envelope protein
  • Alt name
    N-T2A-M-IRES-E
  • Species
    SARS-CoV-2
  • Mutation
    R203M in N protein
  • Entrez Gene
    N (a.k.a. GU280_gp10)
  • Entrez Gene
    E (a.k.a. GU280_gp04)
  • Entrez Gene
    M (a.k.a. GU280_gp05)
  • Promoter CMV, EF-1a

Cloning Information

  • Cloning method Other

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Stelios Andreadis, Plasmid LVDP-CArG-RE-GPR (Addgene # 89762); Jennifer Doudna, Plasmid Luc-PS9 (Addgene # 177942)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LVDP 2P.2 was a gift from Sriram Neelamegham (Addgene plasmid # 231904 ; http://n2t.net/addgene:231904 ; RRID:Addgene_231904)

  • For your References section:

    Conserved role of spike S2 domain N-glycosylation across betacoronaviruses. Yang Q, Kelkar A, Manicassamy B, Neelamegham S. npj Viruses. 2025 Jan; 3(4). doi: 10.1038/s44298-024-00085-7. 10.1038/s44298-024-00085-7
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