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Addgene

pcDNA3.1 N-T2A-M-IRES-E
(Plasmid #231903)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231903 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nucleocapsid protein-T2A-membrane protein-IRES-envelope protein
  • Alt name
    N-T2A-M-IRES-E
  • Species
    SARS-CoV-2
  • Mutation
    R203M in N protein
  • Entrez Gene
    N (a.k.a. GU280_gp10)
  • Entrez Gene
    M (a.k.a. GU280_gp05)
  • Entrez Gene
    E (a.k.a. GU280_gp04)
  • Promoter CMV

Cloning Information

  • Cloning method Other

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Jennifer Doudna, Plasmid CoV2-N-R203M (Addgene # 177952) and PlasmidCoV2-M-IRES-E (Addgene # 177938)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 N-T2A-M-IRES-E was a gift from Sriram Neelamegham (Addgene plasmid # 231903 ; http://n2t.net/addgene:231903 ; RRID:Addgene_231903)

  • For your References section:

    Tuning the tropism and infectivity of SARS-CoV-2 virus-like particles (VLPs) for mRNA delivery. Qi Y, Davidson B, Pajic P, Chen X, Omer G, Min G, Neelamegham S. Nucleic Acids Research, Volume 53, Issue 5, 24 March 2025, gkaf133 10.1093/nar/gkaf133
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