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GFP-polyQ74-NLS
(Plasmid #231892)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231892 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HTT partial exon 1 Q74-NLS
  • Species
    H. sapiens (human)
  • Mutation
    CAG repeat expansion
  • Entrez Gene
    HTT (a.k.a. HD, IT15, LOMARS)
  • Tags / Fusion Proteins
    • EGFP (N terminal on backbone)
    • SV40 NLS (C terminal on insert)

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Internal plasmid ID GK064

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-polyQ74-NLS was a gift from Lukas Kapitein (Addgene plasmid # 231892 ; http://n2t.net/addgene:231892 ; RRID:Addgene_231892)

  • For your References section:

    Nuclear poly-glutamine aggregates rupture the nuclear envelope and hinder its repair. Korsten G, Osinga M, Pelle RA, Serweta AK, Hoogenberg B, Kampinga HH, Kapitein LC. J Cell Biol. 2024 Nov 4;223(11):e202307142. doi: 10.1083/jcb.202307142. Epub 2024 Aug 16. 10.1083/jcb.202307142 PubMed 39150509
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