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Addgene

AAV-DIO-SPOTlight-U2GCR
(Plasmid #231889)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231889 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV
  • Backbone size w/o insert (bp) 7076
  • Total vector size (bp) 3175
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DIO-SPOTlight-U2GCR
  • Alt name
    ISR state-dependent protein synthesis reporter (Cre-dependent)
  • Insert Size (bp)
    3079
  • Promoter CAG

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer TCCTTGAAGAAGATGGTGCG
  • 3′ sequencing primer TCACTTGTTCCTAGGACACGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.10.14.618312 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-DIO-SPOTlight-U2GCR was a gift from Nicole Calakos (Addgene plasmid # 231889 ; http://n2t.net/addgene:231889 ; RRID:Addgene_231889)
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