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Addgene

pDest17_Gcn4 F124I
(Plasmid #231864)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231864 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDest17
  • Backbone manufacturer
    Invtirogen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Gcn4
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    F124I
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis, TEV cleavage site (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositing lab recommends using BL21_pLysS strain for protein expression and purification. Please visit https://doi.org/10.1101/2024.11.21.624739 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDest17_Gcn4 F124I was a gift from Aseem Ansari & Tanja Mittag (Addgene plasmid # 231864 ; http://n2t.net/addgene:231864 ; RRID:Addgene_231864)

  • For your References section:

    Reconciling competing models on the roles of condensates and soluble complexes in transcription factor function. Bremer A, Lang WH, Kempen RP, Sweta K, Taylor AB, Borgia MB, Ansari AZ, Mittag T. bioRxiv 2024.11.21.624739 10.1101/2024.11.21.624739
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