1996_pCCLsin.PPT.hPGK.EGFP-mCEP83.PRE
(Plasmid
#231805)
-
PurposeLabel the mother centrosome-specific distal appendage protein CEP83 by electroporating an mRNA encoding it as well as an eGFP fusion protein.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 231805 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepRRL.hPGK.GP91
-
Modifications to backbonehPGK1 promoter in exchange for GP91
-
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameeGFP-mCEP83
-
SpeciesM. musculus (mouse); Cep83
-
GenBank ID77048
-
Tag
/ Fusion Protein
- eGFP
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made byAimin Liu. For cloning of the plasmid used for eGFP-mCEP83 mRNA production, the eGFP-mCEP83 cassette from plasmid “pEGFPC1-Cep83” (a gift from Aimin Liu, Addgene #128874) was excised and inserted into “pRRL.hPGK.GP91” under control of the hPGK1 promoter and in exchange for GP91.
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
1996_pCCLsin.PPT.hPGK.EGFP-mCEP83.PRE was a gift from Annette Oxenius (Addgene plasmid # 231805 ; http://n2t.net/addgene:231805 ; RRID:Addgene_231805)