1993_pCCLsin.PPT.hPGK.KaedeCETN1.PRE
(Plasmid #231804)

• Purpose: Label centrosomes with lentivirus by encoding a fusion protein consisting of the photo-convertible protein Kaede fused to Centrin 1 (CETN1), an integral component of centrosomes.
• Depositing Lab: Annette Oxenius
• Publication: Targeted Localization of the Mother Centrosome in CD8+ T Cells Undergoing Asymmetric Cell Division Promotes Memory Formation (unpublished)

Backbone

• Vector backbone: pRRL.hPGK.GP91
• Modifications to backbone: hPGK1 promoter in exchange for GP91
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Kaede-CETN1
• Species: M. musculus (mouse)
• Entrez Gene: Cetn1 (a.k.a. caltractin)
• Tag / Fusion Protein:
  • Kaede

Resource Information

• Supplemental Documents:
  • 1993_pCCLsin.PPT.hPGK.KaedeCETN1.PRE.gb
• A portion of this plasmid was derived from a plasmid made by: Germàn Camargo Ortega and Didier Trono. Plasmids for lentivirus production (“pMD2.G” containing VSV-G, “pRSV-Rev” containing Rev, “pMDLg/pRRE” containing Gag and Pol, “pRRL.PPT.SF.KAEDECent1.PRE” containing Kaede-CETN1 and “pAdVAntage™”) were a gift from Germàn Camargo Ortega (ETH Zurich). For cloning of the transfer vector used in this study, Kaede-CETN1 was excised from the transfer vector “pRRL.PPT.SF.KAEDECent1.PRE” and inserted into “pRRL.hPGK.GP91” (a gift from Didier Trono, Addgene #30477) under control of the hPGK1 promoter and in exchange for GP91.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry