pCMV-mGold2s-Keratin-N-17
(Plasmid
#231767)
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PurposeVisualization of Keratin in mammalian cells using mGold2s
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 231767 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
- Backbone size w/o insert (bp) 5994
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemGold2s-Keratin-N-17
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SpeciesH. sapiens (human)
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MutationV282L
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Entrez GeneKRT18 (a.k.a. CK-18, CYK18, K18)
- Promoter CMV
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Tag
/ Fusion Protein
- mGold2s (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-mGold2s-Keratin-N-17 was a gift from Francois St-Pierre (Addgene plasmid # 231767 ; http://n2t.net/addgene:231767 ; RRID:Addgene_231767)