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Addgene

pJL-mGold2t
(Plasmid #231762)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231762 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJL
  • Backbone size w/o insert (bp) 6950
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mGold2t
  • Alt name
    mGold(V1A;V22I;H77N;Q80R;K101E;D117G; I123V;S147C;Y151F;K156R;K158Q;A163V;D173V;S205T;G232S)
  • Alt name
    mVenus(V1A;V22I;L46F;T63S;H77N;Q80R;K101E;D117G; I123V;S147C;Y151F;K156R;K158Q;A163V;D173V;S205T;G232S)
  • Species
    Synthetic
  • Insert Size (bp)
    720
  • Mutation
    mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D117G; I123V;S147C;Y151F;K156R;K158Q;A163V;D173V;S205T;G232S mutations
  • Promoter pTDH(GAP promoter)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer GTAATTCTGTAAATCTATTTCTTAAACTTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJL-mGold2t was a gift from Francois St-Pierre (Addgene plasmid # 231762 ; http://n2t.net/addgene:231762 ; RRID:Addgene_231762)
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