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Addgene

pZZMP09
(Plasmid #231754)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231754 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1 (+)
  • Backbone manufacturer
    Thermo Fisher
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SPO11
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1185
  • Entrez Gene
    Spo11 (a.k.a. AI449549, Spo11a, Spo11b)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.11.20.624382 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZZMP09 was a gift from Scott Keeney (Addgene plasmid # 231754 ; http://n2t.net/addgene:231754 ; RRID:Addgene_231754)

  • For your References section:

    Reconstitution of SPO11-dependent double-strand break formation. Zheng Z, Zheng L, Arter M, Liu K, Yamada S, Ontoso D, Kim S, Keeney S. bioRxiv [Preprint]. 2024 Nov 21:2024.11.20.624382. doi: 10.1101/2024.11.20.624382. 10.1101/2024.11.20.624382 PubMed 39605552
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