pKT2/CAGXSP/GFPCD63
(Plasmid
#231709)
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PurposeStably express CD63 fused with GFP in mammalian cells via the sleeping beauty transposon system.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 231709 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepKT2/CAGXSP
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Backbone manufacturerMichael H. Bachmann
- Backbone size w/o insert (bp) 6800
- Total vector size (bp) 8324
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCD63
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SpeciesH. sapiens (human)
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Insert Size (bp)714
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Entrez GeneCD63 (a.k.a. AD1, HOP-26, ME491, MLA1, OMA81H, Pltgp40, TSPAN30)
- Promoter CAG
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Tag
/ Fusion Protein
- gfp (N terminal on insert)
Cloning Information
- Cloning method Other
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKT2/CAGXSP/GFPCD63 was a gift from Masamitsu Kanada (Addgene plasmid # 231709 ; http://n2t.net/addgene:231709 ; RRID:Addgene_231709) -
For your References section:
Dynamic reorganization of multivesicular bodies and exosome production impacted by sonoporation. Li W, Saleh NA, Gao C, Gagea MA, Vitija X, Kanada M, Deng CX. Sci Rep. 2024 Nov 9;14(1):27432. doi: 10.1038/s41598-024-79042-6. 10.1038/s41598-024-79042-6 PubMed 39521850