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Addgene

pCMV-Nopt
(Plasmid #231704)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 231704 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression ; VSV expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VSV N gene (codon-optimized )
  • Species
    vesicular stomatitis virus
  • Mutation
    human codon-optimized
  • Entrez Gene
    VSIVgp1 (a.k.a. VSIVgp1)
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Nopt was a gift from Ron Geller (Addgene plasmid # 231704 ; http://n2t.net/addgene:231704 ; RRID:Addgene_231704)

  • For your References section:

    An efficient plasmid-based system for the recovery of recombinant vesicular stomatitis virus encoding foreign glycoproteins. Marques MC, Andreu-Moreno I, Sanjuan R, Elena SF, Geller R. Sci Rep. 2024 Jun 25;14(1):14644. doi: 10.1038/s41598-024-65384-8. 10.1038/s41598-024-65384-8 PubMed 38918479
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