pZH82 pET28a-Rai1
(Plasmid
#231651)
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PurposeBacterial expression vector expressing yeast S. cerevisiae rai1, no tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 231651 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZH82 pET28a-Rai1 was a gift from Jesper Svejstrup (Addgene plasmid # 231651 ; http://n2t.net/addgene:231651 ; RRID:Addgene_231651) -
For your References section:
DNA-directed termination of RNA polymerase II transcription. Han Z, Moore GA, Mitter R, Lopez Martinez D, Wan L, Dirac Svejstrup AB, Rueda DS, Svejstrup JQ. Mol Cell. 2023 Sep 21;83(18):3253-3267.e7. doi: 10.1016/j.molcel.2023.08.007. Epub 2023 Sep 7. 10.1016/j.molcel.2023.08.007 PubMed 37683646