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Addgene

pZH81 pET21b Rat1-His
(Plasmid #231650)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231650 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rat1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    RAT1 (a.k.a. YOR048C, HKE1, TAP1, XRN2)
  • Tag / Fusion Protein
    • 6His (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZH81 pET21b Rat1-His was a gift from Jesper Svejstrup (Addgene plasmid # 231650 ; http://n2t.net/addgene:231650 ; RRID:Addgene_231650)

  • For your References section:

    DNA-directed termination of RNA polymerase II transcription. Han Z, Moore GA, Mitter R, Lopez Martinez D, Wan L, Dirac Svejstrup AB, Rueda DS, Svejstrup JQ. Mol Cell. 2023 Sep 21;83(18):3253-3267.e7. doi: 10.1016/j.molcel.2023.08.007. Epub 2023 Sep 7. 10.1016/j.molcel.2023.08.007 PubMed 37683646
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