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Addgene

pZH61 pLIB-8His3C-spt4
(Plasmid #231649)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231649 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLIB
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    spt4
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    SPT4 (a.k.a. YGR063C)
  • Tag / Fusion Protein
    • 8His-HRV3C (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer Polyhedrin promoter, forward primer AAATGATAACCATCTCGC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZH61 pLIB-8His3C-spt4 was a gift from Jesper Svejstrup (Addgene plasmid # 231649 ; http://n2t.net/addgene:231649 ; RRID:Addgene_231649)

  • For your References section:

    DNA-directed termination of RNA polymerase II transcription. Han Z, Moore GA, Mitter R, Lopez Martinez D, Wan L, Dirac Svejstrup AB, Rueda DS, Svejstrup JQ. Mol Cell. 2023 Sep 21;83(18):3253-3267.e7. doi: 10.1016/j.molcel.2023.08.007. Epub 2023 Sep 7. 10.1016/j.molcel.2023.08.007 PubMed 37683646
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